THE NANOIMAGER

The Nanoimager is the complete package in super-resolution microscopy

Nanoimager
  • One microscope, infinite possibilities

    dSTORM, PALM, single-particle tracking, SIM, Confocal, TIRF, HILO, smFRET – the Nanoimager means multiple microscopy techniques at your command.

  • What will you do with your optical table?

    You won't need one with the Nanoimager. Its in-built dampening counters vibrations. You can also forget about alignment. It's already been taken care of, for ever.

  • Integrated analytics, fast

    With integrated analysis tools, you start seeing results before the experiment has even finished.

  • Lasers kept in, background kept out

    The Nanoimager is designed to keep its lasers in, and ambient light out so you won’t need a dark room. Coupled with its compact size, you can locate it wherever it’s most productive.

  • Panoramic field of view

    At 80µm x 50µm, the field of view is one of the largest available in super-resolution. Combine it with a piezo stage working in 2nm steps, and you can automatically stitch overview scans for a total view of the sample.

  • Four colors, Two at once

    With four laser colors, four different fluorophores can be analysed in each sample. And two can be captured simultaneously to give a deeper understanding of how different molecules interact.

UNDER THE HOOD

Compact yet complete

We are scientists ourselves. So we have thought about the features that make life easier.

  • Microfluidics compatible

  • Temperature control to 42℃

  • Drift correction

  • Real-time autofocus

  • High laser power density

  • Unlimited licences for analysis software

  • Class 1 laser product

  • LED array for brightfield illumination

  • Piezo stage with 2nm step size

HARDWARE OPTIONS

Compare Models

The Nanoimager comes in two formats. Here, we outline the key differences between the S and X models.

Nanoimager S

Nanoimager S

Nanoimager X

Nanoimager X

  • Number of Imaging Colors

    Up to 4 lasers

    Up to 3 lasers

  • Simultaneous channels

    2 channels

    Up to 2 channels

  • Single Molecule Localization Microscopy

    3D imaging

    3D imaging optional

  • Camera

    1.6 electrons rms read noise

    2.3 electrons rms read noise

  • Light Engine Dimensions

    215mm x 420mm x 450mm

    215mm x 420mm x 250mm

  • SIM and Confocal

    As standard

    Optional

Full Specifications:

NANOIMAGER S
IMAGING AND ANALYSIS

Imaging modalities

  • Single-molecule imaging based 3D localization microscopy

  • Förster resonance energy transfer (FRET) spectroscopy

  • Single-molecule tracking

  • SIM / Confocal

  • Epifluorescence / HILO / TIRF illumination

Software features

  • Real-time 3D localization analysis and rendering (sCMOS optimized)

  • FRET trace analysis

  • Residual drift correction

  • Scripting interface

Achievable resolution

  • Lateral: exceeding 20nm

  • Axial: exceeding 50nm

Acquistion speed

  • 100 fps full frame

  • 2500 fps with frame height cropped to 2%

Simultaneous dual-color channels

  • <10nm channel mapping accuracy

3D imaging technique

  • Astigmatism

Total number of imaging colors

  • Up to 4 lasers

Field of view

  • 50µm x 80µm per channel

NANOIMAGER S
OPERATIONAL

Focus system

  • Manual Z-reference selection

  • One-shot autofocus

  • Continuous autofocus

Mechanical stability

  • <1µm/K drift

  • <1nm vibration amplitude (1 Hz to 500 Hz)

Integrated sensors

  • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection

  • Closed-loop adjustments of laser power density at sample plane

Temperature control

  • Resistive heating, whole instrument (for live cell imaging)

Environmental conditions

  • Sensor array (temperature, humidity, acceleration)

NANOIMAGER S
HARDWARE

Dimensions W x D x H

  • Microscope: 215mm x 215mm x 155mm

  • Light engine: 215mm x 420mm x 450mm

Camera

  • sCMOS

  • 82% peak QE

  • 1.6 electrons rms read noise at standard scan

Objective

  • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion

  • 60x, 1.42NA, oil immersion

Laser options

  • Violet: 405nm (150mW or 1000mW)

  • Blue: 473nm (300mW or 1000mW), 488nm (200mW or 1000mW)

  • Green: 532nm (300mW or 1000mW), 561nm (200mW or 500mW or 1000mW)

  • Red: 640nm (300mW or 1000mW)

  • Far red: 720nm (1200mW)

Laser types

  • DPSS and diode

Other light sources

  • LED for bright-field imaging

  • NIR auto-focus laser

Sample stage

  • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1nm encoder resolution

PC requirements

  • Laptop included (32GB RAM, nVidia GeForce GTX 1080M)

  • Nanoimager software included with unlimited license

  • Free regular software updates

NANOIMAGER X
IMAGING AND ANALYSIS

Imaging modalities

  • Single-molecule imaging based 2D localization microscopy (3D optional)

  • Förster resonance energy transfer (FRET) spectroscopy

  • Single-molecule tracking

  • SIM / Confocal (optional)

  • Epifluorescence / HILO / TIRF illumination

Software features

  • Real-time 2D localization analysis and rendering (sCMOS optimized)

  • FRET trace analysis

  • Residual drift correction

  • Scripting interface

Achievable resolution

  • Lateral: exceeding 20nm

  • Axial: exceeding 50nm

Acquistion speed

  • 100 fps full frame

  • 2500 fps with frame height cropped to 2%

Single channel (2 optional)

  • <10nm channel mapping accuracy

3D imaging technique

  • Astigmatism (optional)

Total number of imaging colors

  • Up to 3 lasers

Field of view

  • 50µm x 80µm per channel

NANOIMAGER X
OPERATIONAL

Focus system

  • Manual Z-reference selection

  • One-shot autofocus

  • Continuous autofocus

Mechanical stability

  • <1µm/K drift

  • <1nm vibration amplitude (1 Hz to 500 Hz)

Integrated sensors

  • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection

  • Closed-loop adjustments of laser power density at sample plane

Temperature control

  • Resistive heating, whole instrument (for live cell imaging)

Environmental conditions

  • Sensor array (temperature, humidity, acceleration)

NANOIMAGER X
HARDWARE

Dimensions W x D x H

  • Microscope: 215mm x 215mm x 155mm

  • Light engine: 215mm x 420mm x 250mm

Camera

  • sCMOS

  • 82% peak QE

  • 2.3 electrons rms read noise at standard scan

Objective

  • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion

  • 60x, 1.42NA, oil immersion

Laser options

  • Violet: 405nm (150mW or 1000mW)

  • Blue: 473nm (300mW or 1000mW), 488nm (200mW or 1000mW)

  • Green: 532nm (300mW or 1000mW), 561nm (200mW or 500mW or 1000mW)

  • Red: 640nm (300mW or 1000mW)

  • Far red: 720nm (1200mW)

Laser types

  • DPSS and diode

Other light sources

  • LED for bright-field imaging

  • NIR auto-focus laser

Sample stage

  • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1nm encoder resolution

PC requirements

  • Laptop included (32GB RAM, nVidia GeForce GTX 1080M)

  • Nanoimager software included with unlimited license

  • Free regular software updates

HARDWARE OPTIONS

Compare Models

The Nanoimager comes in two formats. Here, we outline the key differences between the the S and X models.

  • Number of Imaging Colors

    Up to 4 lasers
  • Simultaneous channels

    2 channels
  • Single Molecule Localization Microscopy

    3D imaging
  • Camera

    1.6 electrons rms read noise
  • Light Engine Dimensions

    215mm x 420mm x 450mm
  • SIM and Confocal

    As standard
  • Number of Imaging Colors

    Up to 3 lasers
  • Simultaneous channels

    Up to 2 channels
  • Single Molecule Localization Microscopy

    3D imaging optional
  • Camera

    2.3 electrons rms read noise
  • Light Engine Dimensions

    215mm x 420mm x 250mm
  • SIM and Confocal

    Optional

Full Specifications:

  • NANOIMAGER S
    IMAGING AND ANALYSIS Icon

    Imaging modalities

    • Single-molecule imaging based 3D localization microscopy
    • Förster resonance energy transfer (FRET) spectroscopy
    • Single-molecule tracking
    • SIM / Confocal
    • Epifluorescence / HILO / TIRF illumination

    Software features

    • Real-time 3D localization analysis and rendering (sCMOS optimized)
    • FRET trace analysis
    • Residual drift correction
    • Scripting interface

    Achievable resolution

    • Lateral: exceeding 20nm
    • Axial: exceeding 50nm

    Acquistion speed

    • 100 fps full frame
    • 2500 fps with frame height cropped to 2%

    Simultaneous dual-color channels

    • <10nm channel mapping accuracy

    3D imaging technique

    • Astigmatism

    Total number of imaging colors

    • Up to 4 lasers

    Field of view

    • 50µm x 80µm per channel
  • NANOIMAGER S
    OPERATIONAL Icon

    Focus system

    • Manual Z-reference selection
    • One-shot autofocus
    • Continuous autofocus

    Mechanical stability

    • <1µm/K drift
    • <1nm vibration amplitude (1 Hz to 500 Hz)

    Integrated sensors

    • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
    • Closed-loop adjustments of laser power density at sample plane

    Temperature control

    • Resistive heating, whole instrument (for live cell imaging)

    Environmental conditions

    • Sensor array (temperature, humidity, acceleration)
  • NANOIMAGER S
    HARDWARE Icon

    Dimensions W x D x H

    • Microscope: 215mm x 215mm x 155mm
    • Light engine: 215mm x 420mm x 450mm

    Camera

    • sCMOS
    • 82% peak QE
    • 1.6 electrons rms read noise at standard scan

    Objective

    • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion
    • 60x, 1.42NA, oil immersion

    Laser options

    • Violet: 405nm (150mW or 1000mW)
    • Blue: 473nm (300mW or 1000mW), 488nm (200mW or 1000mW)
    • Green: 532nm (300mW or 1000mW), 561nm (200mW or 500mW or 1000mW)
    • Red: 640nm (300mW or 1000mW)
    • Far red: 720nm (1200mW)

    Laser types

    • DPSS and diode

    Other light sources

    • LED for bright-field imaging
    • NIR auto-focus laser

    Sample stage

    • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1nm encoder resolution

    PC requirements

    • Laptop included (32GB RAM, nVidia GeForce GTX 1080M)
    • Nanoimager software included with unlimited license
    • Free regular software updates
  • NANOIMAGER X
    IMAGING AND ANALYSIS Icon

    Imaging modalities

    • Single-molecule imaging based 2D localization microscopy (3D optional)
    • Förster resonance energy transfer (FRET) spectroscopy
    • Single-molecule tracking
    • SIM / Confocal (optional)
    • Epifluorescence / HILO / TIRF illumination

    Software features

    • Real-time 2D localization analysis and rendering (sCMOS optimized)
    • FRET trace analysis
    • Residual drift correction
    • Scripting interface

    Achievable resolution

    • Lateral: exceeding 20nm
    • Axial: exceeding 50nm

    Acquistion speed

    • 100 fps full frame
    • 2500 fps with frame height cropped to 2%

    Single channel (2 optional)

    • <10nm channel mapping accuracy

    3D imaging technique

    • Astigmatism (optional)

    Total number of imaging colors

    • Up to 3 lasers

    Field of view

    • 50µm x 80µm per channel
  • NANOIMAGER X
    OPERATIONAL Icon

    Focus system

    • Manual Z-reference selection
    • One-shot autofocus
    • Continuous autofocus

    Mechanical stability

    • <1µm/K drift
    • <1nm vibration amplitude (1 Hz to 500 Hz)

    Integrated sensors

    • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
    • Closed-loop adjustments of laser power density at sample plane

    Temperature control

    • Resistive heating, whole instrument (for live cell imaging)

    Environmental conditions

    • Sensor array (temperature, humidity, acceleration)
  • NANOIMAGER X
    HARDWARE Icon

    Dimensions W x D x H

    • Microscope: 215mm x 215mm x 155mm
    • Light engine: 215mm x 420mm x 250mm

    Camera

    • sCMOS
    • 82% peak QE
    • 2.3 electrons rms read noise at standard scan

    Objective

    • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion
    • 60x, 1.42NA, oil immersion

    Laser options

    • Violet: 405nm (150mW or 1000mW)
    • Blue: 473nm (300mW or 1000mW), 488nm (200mW or 1000mW)
    • Green: 532nm (300mW or 1000mW), 561nm (200mW or 500mW or 1000mW)
    • Red: 640nm (300mW or 1000mW)
    • Far red: 720nm (1200mW)

    Laser types

    • DPSS and diode

    Other light sources

    • LED for bright-field imaging
    • NIR auto-focus laser

    Sample stage

    • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1nm encoder resolution

    PC requirements

    • Laptop included (32GB RAM, nVidia GeForce GTX 1080M)
    • Nanoimager software included with unlimited license
    • Free regular software updates

Download Full Technical Specification

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