THE NANOIMAGER

The Nanoimager is
the complete package in
super-resolution microscopy

Nanoimager
  • One microscope, infinite possibilities

    dSTORM, PALM, single-particle tracking, SIM, Confocal, TIRF, smFRET – the Nanoimager means multiple microscopy techniques at your command.

  • What will you do with your optical table?

    You won’t need one with the Nanoimager. Its in-built dampening counters vibrations. You can also forget about alignment. It's already been taken care of, for ever.

  • Integrated analytics, fast

    With integrated analysis tools, you start seeing results before the experiment has even finished.

  • Lasers kept in, background kept out

    The Nanoimager is designed to keep its lasers in, and ambient light out so you won’t need a dark room. Coupled with its compact size, you can locate it wherever it’s most productive.

  • Panoramic field of view

    At 80µm x 50µm, the field of view is one of the largest available in super-resolution. Combine it with a piezo stage working in 2nm steps, and you can automatically stitch overview scans for a total view of the sample.

  • Four colors, Two at once

    With four laser colors, four different fluorophores can be analysed in each sample. And two can be captured simultaneously to give a deeper understanding of how different molecules interact.

UNDER THE HOOD

Compact yet complete

We're scientists ourselves. So we've thought about the features that make life easier.

  • Microfluidics compatible
  • Temperature control to 42℃
  • Drift correction
  • Real-time autofocus
  • High laser power density
  • Unlimited licences for analysis software
  • Class 1 laser product
  • LED array for brightfield illumination
  • Peizo stage with 2 nm step size
Nanoimager

Techniques and Illumination Modes

View Techniques

Advanced Software: NimOS

Explore Software

Applications

View Case Studies

HARDWARE OPTIONS

Compare Models

The Nanoimager comes in two formats. Here, we outline the key differences between the the S and X models.

Nanoimager S

Nanoimager S

Nanoimager X

Nanoimager X

  • Number of Imaging Colours

    Up to 4 lasers

    Up to 3 lasers

  • Simultaneous channels

    2 channels

    Up to 2 channels

  • Single Molecule Localisation Microscopy

    3D imaging

    3D imaging optional

  • Camera

    1.6 electrons rms read noise

    2.3 electrons rms read noise

  • Light Engine Dimensions

    21cm x 42cm x 45cm

    21cm x 42cm x 25cm

  • SIM and Confocal

    As standard

    Optional

Full Specifications:

NANOIMAGER S
IMAGING AND ANALYSIS

Imaging modalities

  • Single-molecule imaging based 3D localization microscopy
  • Förster resonance energy transfer (FRET) spectroscopy
  • Single-molecule tracking
  • SIM / Confocal
  • Epifluorescence / HILO / TIRF illumination

Software features

  • Real-time 3D localization analysis and rendering (sCMOS optimized)
  • FRET trace analysis
  • Residual drift correction
  • Scripting interface

Achievable resolution

  • Lateral: exceeding 20 nm
  • Axial: exceeding 50 nm

Acquistion speed

  • 100 fps full frame
  • 2500 fps with frame height cropped to 2%

Simultaneous dual-color channels

  • < 10 nm channel mapping accuracy

3D imaging technique

  • Astigmatism

Total number of imaging colors

  • Up to 4 lasers

Field of view

  • 50µm x 80µm per channel

NANOIMAGER S
OPERATIONAL

Focus system

  • Manual Z-reference selection
  • One-shot autofocus
  • Continuous autofocus

Mechanical stability

  • <1µm/K drift
  • <1 nm vibration amplitude (1 Hz to 500 Hz)

Integrated sensors

  • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
  • Closed-loop adjustments of laser power density at sample plane

Temperature control

  • Resistive heating, whole instrument (for live cell imaging)

Environmental conditions

  • Sensor array (temperature, humidity, acceleration)

NANOIMAGER S
HARDWARE

Dimensions W x D x H

  • Microscope: 21cm x 21cm x 15cm
  • Light engine: 21cm x 42cm x 45cm

Camera

  • sCMOS
  • 82% peak QE
  • 1.6 electrons rms read noise at standard scan

Objective

  • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion
  • 60x, 1.42NA, oil immersion

Laser options

  • Violet: 405 nm (150 mW or 1000 mW)
  • Blue: 473 nm (300 mW or 1000 mW), 488 nm (200 mW or 1000 mW)
  • Green: 532 nm (300 mW or 1000 mW), 561 nm (200 mW or 500 mW or 1000 mW)
  • Red: 640 nm (300 mW or 1000 mW)
  • Far red: 720 nm (1200 mW)

Laser types

  • DPSS and diode

Other light sources

  • LED for bright-field imaging
  • NIR auto-focus laser

Sample stage

  • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution

PC requirements

  • Laptop included (32 GB RAM, nVidia GeForce GTX 1080M)
  • Nanoimager software included with unlimited license
  • Free regular software updates

NANOIMAGER X
IMAGING AND ANALYSIS

Imaging modalities

  • Single-molecule imaging based 2D localization microscopy (3D optional)
  • Förster resonance energy transfer (FRET) spectroscopy
  • Single-molecule tracking
  • SIM / Confocal (optional)
  • Epifluorescence / HILO / TIRF illumination

Software features

  • Real-time 2D localization analysis and rendering (sCMOS optimized)
  • FRET trace analysis
  • Residual drift correction
  • Scripting interface

Achievable resolution

  • Lateral: exceeding 20 nm
  • Axial: exceeding 50 nm

Acquistion speed

  • 100 fps full frame
  • 2500 fps with frame height cropped to 2%

Single channel (2 optional)

  • < 10 nm channel mapping accuracy

3D imaging technique

  • Astigmatism (optional)

Total number of imaging colors

  • Up to 3 lasers

Field of view

  • 50µm x 80µm per channel

NANOIMAGER X
OPERATIONAL

Focus system

  • Manual Z-reference selection
  • One-shot autofocus
  • Continuous autofocus

Mechanical stability

  • <1µm/K drift
  • <1 nm vibration amplitude (1 Hz to 500 Hz)

Integrated sensors

  • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
  • Closed-loop adjustments of laser power density at sample plane

Temperature control

  • Resistive heating, whole instrument (for live cell imaging)

Environmental conditions

  • Sensor array (temperature, humidity, acceleration)

NANOIMAGER X
HARDWARE

Dimensions W x D x H

  • Microscope: 21cm x 21cm x 15cm
  • Light engine: 21cm x 42cm x 25cm

Camera

  • sCMOS
  • 82% peak QE
  • 2.3 electrons rms read noise at standard scan

Objective

  • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion
  • 60x, 1.42NA, oil immersion

Laser options

  • Violet: 405 nm (150 mW or 1000 mW)
  • Blue: 473 nm (300 mW or 1000 mW), 488 nm (200 mW or 1000 mW)
  • Green: 532 nm (300 mW or 1000 mW), 561 nm (200 mW or 500 mW or 1000 mW)
  • Red: 640 nm (300 mW or 1000 mW)
  • Far red: 720 nm (1200 mW)

Laser types

  • DPSS and diode

Other light sources

  • LED for bright-field imaging
  • NIR auto-focus laser

Sample stage

  • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution

PC requirements

  • Laptop included (32 GB RAM, nVidia GeForce GTX 1080M)
  • Nanoimager software included with unlimited license
  • Free regular software updates

HARDWARE OPTIONS

Compare Models

The Nanoimager comes in two formats. Here, we outline the key differences between the the S and X models.

  • Number of Imaging Colours

    Up to 4 lasers
  • Simultaneous channels

    2 channels
  • Single Molecule Localisation Microscopy

    3D imaging
  • Camera

    1.6 electrons rms read noise
  • Light Engine Dimensions

    21cm x 42cm x 45cm
  • SIM and Confocal

    As standard
  • Number of Imaging Colours

    Up to 3 lasers
  • Simultaneous channels

    Up to 2 channels
  • Single Molecule Localisation Microscopy

    3D imaging optional
  • Camera

    2.3 electrons rms read noise
  • Light Engine Dimensions

    21cm x 42cm x 25cm
  • SIM and Confocal

    Optional

Full Specifications:

  • NANOIMAGER S
    IMAGING AND ANALYSIS Icon

    Imaging modalities

    • Single-molecule imaging based 3D localization microscopy
    • Förster resonance energy transfer (FRET) spectroscopy
    • Single-molecule tracking
    • SIM / Confocal
    • Epifluorescence / HILO / TIRF illumination

    Software features

    • Real-time 3D localization analysis and rendering (sCMOS optimized)
    • FRET trace analysis
    • Residual drift correction
    • Scripting interface

    Achievable resolution

    • Lateral: exceeding 20 nm
    • Axial: exceeding 50 nm

    Acquistion speed

    • 100 fps full frame
    • 2500 fps with frame height cropped to 2%

    Simultaneous dual-color channels

    • < 10 nm channel mapping accuracy

    3D imaging technique

    • Astigmatism

    Total number of imaging colors

    • Up to 4 lasers

    Field of view

    • 50µm x 80µm per channel
  • NANOIMAGER S
    OPERATIONAL Icon

    Focus system

    • Manual Z-reference selection
    • One-shot autofocus
    • Continuous autofocus

    Mechanical stability

    • <1µm/K drift
    • <1 nm vibration amplitude (1 Hz to 500 Hz)

    Integrated sensors

    • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
    • Closed-loop adjustments of laser power density at sample plane

    Temperature control

    • Resistive heating, whole instrument (for live cell imaging)

    Environmental conditions

    • Sensor array (temperature, humidity, acceleration)
  • NANOIMAGER S
    HARDWARE Icon

    Dimensions W x D x H

    • Microscope: 21cm x 21cm x 15cm
    • Light engine: 21cm x 42cm x 45cm

    Camera

    • sCMOS
    • 82% peak QE
    • 1.6 electrons rms read noise at standard scan

    Objective

    • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion
    • 60x, 1.42NA, oil immersion

    Laser options

    • Violet: 405 nm (150 mW or 1000 mW)
    • Blue: 473 nm (300 mW or 1000 mW), 488 nm (200 mW or 1000 mW)
    • Green: 532 nm (300 mW or 1000 mW), 561 nm (200 mW or 500 mW or 1000 mW)
    • Red: 640 nm (300 mW or 1000 mW)
    • Far red: 720 nm (1200 mW)

    Laser types

    • DPSS and diode

    Other light sources

    • LED for bright-field imaging
    • NIR auto-focus laser

    Sample stage

    • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution

    PC requirements

    • Laptop included (32 GB RAM, nVidia GeForce GTX 1080M)
    • Nanoimager software included with unlimited license
    • Free regular software updates
  • NANOIMAGER X
    IMAGING AND ANALYSIS Icon

    Imaging modalities

    • Single-molecule imaging based 2D localization microscopy (3D optional)
    • Förster resonance energy transfer (FRET) spectroscopy
    • Single-molecule tracking
    • SIM / Confocal (optional)
    • Epifluorescence / HILO / TIRF illumination

    Software features

    • Real-time 2D localization analysis and rendering (sCMOS optimized)
    • FRET trace analysis
    • Residual drift correction
    • Scripting interface

    Achievable resolution

    • Lateral: exceeding 20 nm
    • Axial: exceeding 50 nm

    Acquistion speed

    • 100 fps full frame
    • 2500 fps with frame height cropped to 2%

    Single channel (2 optional)

    • < 10 nm channel mapping accuracy

    3D imaging technique

    • Astigmatism (optional)

    Total number of imaging colors

    • Up to 3 lasers

    Field of view

    • 50µm x 80µm per channel
  • NANOIMAGER X
    OPERATIONAL Icon

    Focus system

    • Manual Z-reference selection
    • One-shot autofocus
    • Continuous autofocus

    Mechanical stability

    • <1µm/K drift
    • <1 nm vibration amplitude (1 Hz to 500 Hz)

    Integrated sensors

    • Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection
    • Closed-loop adjustments of laser power density at sample plane

    Temperature control

    • Resistive heating, whole instrument (for live cell imaging)

    Environmental conditions

    • Sensor array (temperature, humidity, acceleration)
  • NANOIMAGER X
    HARDWARE Icon

    Dimensions W x D x H

    • Microscope: 21cm x 21cm x 15cm
    • Light engine: 21cm x 42cm x 25cm

    Camera

    • sCMOS
    • 82% peak QE
    • 2.3 electrons rms read noise at standard scan

    Objective

    • 100x, 1.4NA or 1.49NA, oil, silicone or water immersion
    • 60x, 1.42NA, oil immersion

    Laser options

    • Violet: 405 nm (150 mW or 1000 mW)
    • Blue: 473 nm (300 mW or 1000 mW), 488 nm (200 mW or 1000 mW)
    • Green: 532 nm (300 mW or 1000 mW), 561 nm (200 mW or 500 mW or 1000 mW)
    • Red: 640 nm (300 mW or 1000 mW)
    • Far red: 720 nm (1200 mW)

    Laser types

    • DPSS and diode

    Other light sources

    • LED for bright-field imaging
    • NIR auto-focus laser

    Sample stage

    • 20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution

    PC requirements

    • Laptop included (32 GB RAM, nVidia GeForce GTX 1080M)
    • Nanoimager software included with unlimited license
    • Free regular software updates

Need Alterations?

Not every design aspect is fixed in stone and we may have the ability to customise some features to suit your individual needs. Get in touch with one of our scientists to discuss further.