Introducing Automated Super-Resolution Press Release
Aplo Flow
Automation to accelerate discovery
Aplo Flow
The ecosystem for simplified fluidic automation
Aplo Flow combines automated sample preparation with programmable fluidics to deliver reliable, end-to-end super-resolution workflows. Integrated with the Aplo Platform, it minimizes hands-on time, improves reproducibility, and streamlines applications from DNA-PAINT and exchange imaging to extracellular vesicle (EV) and lipid nanoparticle (LNP) characterization.
Enhanced throughput and modular architecture
Aplo Flow’s modular architecture decouples sample preparation from imaging, enabling parallel workflows for greater throughput. Aplo Flow prepares up to 16 samples in 4.5 hours with up to 85% less hands-on time, powering scalable DNA-PAINT experiments and standardized sample-to-answer workflows for EV and LNP research.
One system, two modes of operation
Automated sample preparation
Automate parallel sample preparation directly on the Aplo Flow deck. Process up to 16 samples with up to 85% less hands-on time while improving reproducibility and reducing time to results. Combined with ONI Application Kits™, Aplo Flow delivers complete sample-to-answer workflows for quantitative single-particle characterization of LNPs and EVs.
- Characterize up to 16 LNP samples/day
- Quantify size, cargo loading, integrity, and ligand abundance
- Characterize up to 12 EV samples/day
- Measure EV heterogeneity at the single-particle level
Boundless fluidic microscopy
Aplo Flow transforms complex super-resolution assays into fully automated, reproducible workflows. Programmable fluid handling and proprietary microfluidic consumables enable scalable DNA-PAINT, spatial biology, live-cell uptake, and dosing studies with minimal hands-on intervention. By integrating fluidics, imaging, and analysis into one platform, Aplo Flow delivers reliable unattended operation without compromising image quality or repeatability.
Engineered thermal control
Long-duration imaging with exceptional drift stability.
Purpose-built consumables
Seamless setup with reliable fluid exchange.
Flexible workflow software
Create, automate, and scale complex assays.
Key features
Maximized throughput
Decoupled fluidics and imaging increases speed, processing up to 16 samples in one day
Automated fluidics
Hands-off operation that eliminates manual error and experimental variance
Standardized workflows
Seamless sample-to-answer nanoparticle characterization workflows
Unified platform
Fully automates complex microscopy, infeasible without integrated fluidics
Discover
Aplo Flow
Aplo Flow
ONI Application Kits™
Reagent kits for visualization and characterization of EV or LNP samples with single-particle sensitivity
Pipettor
Provides precise, automated fluid handling, enhancing efficiency and reproducibility
Chip holder
Custom ONI fluidics chip holder (four positions)
Dual tip rack storage
Sufficient tips for uninterrupted preparation of up to 16 samples
Waste capture station
Collects used tips and sample waste for disposal
Custom fluidics pass-through
Fluidic tether pass through for sequential reagent perfusion
Light safe windows
Helps to block harmful light from damaging samples
Custom reagent holder
Workflow specific labware positions for intuitive deck loading
Fluidics driver
All-in-one hardware to automate almost any fluidic-based assay
Transfer station
Scalable, parallel sample processing, including on deck temperature control for sensitive reagents
Get a closer look
Explore
Providing tools to make super-resolution microscopy more accessible.
FAQs
How does Aplo Flow improve workflow efficiency?
Aplo Flow enhances workflow efficiency by fully automating sample preparation for any of ONIs custom reagent kits. With less than 30 minutes of hands-on time, even a novice scientist can prepare up to 16 samples with high reliability and consistency. Instead of spending hours preparing samples by hand, Aplo Flow users are free to image other samples, analyze yesterday’s dataset, or plan their next experiment. More answers in less time; the true meaning of efficiency.
How many samples can Alpo flow process per run?
Process anywhere from 1 to 16 samples using ONI’s particle characterization kits (3x chips with 4x lanes on each), indicating custom experimental conditions for each lane before starting the experiment. Each ONI Cell Flow Chip can accommodate up to 4 samples in a single automated fluidics assay.
How adaptable is Aplo Flow to different research needs?
Aplo Flow’s modular design provides sample level flexibility control in both end-to-end particle characterization workflows and unparalleled versatility for bespoke fluidic assays. Combined, Aplo Flow can cater to virtually limitless experimental designs, while ensuring adaptability and robust scalability in a diverse set of scientific applications.
What support and resources are available for users of Aplo Flow?
Users of Aplo Flow have access to comprehensive support and resources, including training materials, technical assistance, and software updates. This ensures that researchers can maximize the capabilities of Aplo Flow and achieve their scientific goals efficiently and effectively.
Can I use Aplo Flow to build DNA-PAINT workflows?
Yes, the Aplo Platform is designed to accommodate complex assay types exactly like DNA PAINT and exchange PAINT. The Aplo SDK paired with robust Aplo Flow fluidics hardware is well-suited to execute assay types that are typically too tedious to conduct manually, due the need for repeated user intervention over many hours. With Aplo, you can design an experiment in basic python code, place the necessary reagents on the Aplo Flow deck, and trust the Aplo Platform to handle the rest. DNA PAINT experiments with 10+ exchange cycles across up to four (4) samples can be easily automated and run “lights-out”.