Introducing Aplo Scope: A new era in super-resolution imaging! learn more >
Aplo Scope
Simple. Engineered. Dynamic. Powerful.
A new era in super-resolution imaging
Meet Aplo Scope – ONI's new single-molecule super-resolution microscope designed for effortless molecular imaging. Perfect for researchers of all levels, Aplo Scope combines advanced imaging, precise laser control, and intuitive software to deliver high-quality results, from research on the foundations of life to drug discovery, biomarker research, and disease studies.
With its compact design, Aplo Scope transitions seamlessly from live-cell to super-resolution imaging at 15 nm resolution, without complex setup. Its unparalleled ease of use and integration with the Aplo Platform make it ideal for uncovering molecular interactions and driving breakthroughs.
Simple
Designed to simplify complex microscopy with one easy-to-use tool. Start imaging quickly, generate data with ease, and unlock answers quicker than ever before.
Engineered
Robustly engineered for stability, consistency, and reliability, to maximize reproducibility across experiments and users.
Dynamic
Seamlessly transition between live-cell imaging and super-resolution without reconfiguration for unmatched flexibility in one platform.
Powerful
Compact footprint, yet powerful enough to enable complex scientific inquiry. Aplo Scope is designed to fit into your lab, not the other way around.
The new and groundbreaking SMLM microscope
Maximize Throughput
Expansive field-of-view
Aplo Scope contains a 110 by 110 micrometres field-of-view, in both diffraction-limited and single-molecule localization microscopy. The expansive field-of-view significantly increases the chances of capturing rare events and elusive phenotypes.
Detect ultra-rare cell populations and particle biomarkers with unmatched throughput, accelerating groundbreaking discoveries and driving transformative breakthroughs.
More Channels, More Insights
Multicolor imaging reveals biological context
Achieve maximal spectral separation with minimized cross-talk and background thanks to an optimized filter set including single, dual, and quad options. Using only 4 lasers, you can generate detailed multi-color panels of 5 or more labels to elucidate complex protein and particle interactions that span both large pan-cellular structures and nanoscale molecular features.
Leverage the rapid laser switching with multiband filters for nearly simultaneous multi-channel imaging.
Flexibility Redefined
Live cell to super-resolution imaging
Effortlessly capture dynamic cellular events in real-time, then zoom into molecular details with super-resolution precision — all within a single, intuitive platform designed to enable your cutting-edge science. From low-power live-cell imaging to high-power super-resolution imaging, the Aplo Scope eliminates the need for hardware reconfiguration.
Aplo Scope includes unique illumination stability, even at extremely low power (<0.01 kW/cm2), to minimize phototoxicity in extended live cell imaging sessions. At the other end, high laser powers enable rapid, super-resolution imaging to uncover molecular level details.
Homogeneous Illumination
Variation that reflects biology
Aplo Scope produces a consistently flat, symmetric illumination across the entire field of view, vital for statistical and comparative intensity analysis. With consistent resolution and precision edge to edge, every frame produces more data, maximizing throughput of critical and sensitive assays. This enables quantitative molecular studies to better understand crucial mechanisms in disease and drug discovery studies.
Key features
of Aplo Scope
of Aplo Scope
Next-gen lasers
Power density continuously adjustable from 0.01 through 4 kW/cm2
Light engine
Packed with technology to enable diffraction-limited and SMLM imaging
Illumination
Illumination angle control from Epi to HILO and TIRF. Consistent symmetric illumination across the field-of-view
Temperature control
Precise temperature control up to 40°C, resisting ambient environmental fluctuations of up to 3°C
Filter Wheel
Four laser channels can be interlaced with any combination of emission filters to perform multicolor imaging, easily generating 5-color overlays
Camera
Sensitive next-gen CMOS camera for low noise, high-speed imaging
Focus Z-lock
Rapid and precise focus with locking technology to minimize Z-drift
Field-Of-View
110 by 110 micrometres in both diffraction-limited and single-molecule microscopy
Stage
Fully automated piezo stage fits most microscopy slides and plates
Mechanical stability
Integral dual-stage isolation system minimizes vibration
Sample enclosure
Integral lid interlock ensures user safety, class 1 laser instrument
Webinar: Meet Aplo Scope
Therapeutic discoveries
The platform designed for therapeutic discovery
Accelerate transformative research
Visualize unparalleled detail, enabling you to uncover new drug targets and therapeutic pathways.
Quantitative insights
Translate biological data into quantitive insights with advanced analysis and reporting tools.
Aplo Scope gallery
5 color expanded mosaic - 2 channel dSTORM of mitochondria labeled with Tomm20 and AZ647 secondary antibody (yellow) and lysosome stained for LAMP1 and CF583R secondary antibody (red) combined with 3 channels of diffraction limited labeling of nucleus stained with Hoechst (magenta), cell membrane stained with CellMask Green (gray) and tubulin and CF405L secondary antibody (cyan).
Single color dSTORM labeling of nuclear pores stained for Nup98 using AZ647 secondary antibody showing individual pore subunits.
Single color dSTORM of tubulin and AZ647 secondary antibody.
1 channel dSTORM of nuclear pores stained for Nup98 using AZ647 secondary antibody (cyan) combined diffraction limited with a Hoescht nuclear stain (magenta).
2 channel dSTORM of lysosomes with LAMP1 and CF583R secondary antibody (red) and mitochondria stained with Tomm20 and AZ647 secondary antibody (yellow) combined with diffraction limited labeling of tubulin and CF405L secondary antibody (cyan) and cell membrane stained with Cell Mask Green (gray).
Single color DNA PAINT labeling of vimentin with detailed highlights of individual sections at 50% magnification.
Single color dSTORM labeling of tubulin and AZ647 secondary antibody (gray) combined with single color diffraction limited labeling of the nucleus with Hoescht (magenta).
1 color dSTORM labeling of mitochondria stained with Tomm20 and AZ647 secondary antibody (yellow) combined with 4 color diffraction limited labeling of lysosomes with LAMP1 and CF583R secondary antibody (red), nucleus stained with Hoescht (blue), cell membrane stained with CellMask Green (gray), and tubulin and CF405L secondary antibody (cyan).
The Aplo Platform: from sample to answer
Reagent Kits
Discover our kits to get sample preparation sorted: Training Kits and Discovery Kits to help you master the basics of dSTORM imaging and apply them to your own samples, and Application Kits such as EV Profiler 2.
Every bioware kit, hardware tool, and software solution are designed to be compatible, and optimized for consistent performance in any user’s hands.
Aplo Flow
Aplo Flow combines application-specific sample preparation with fully automated fluidic control, enhancing the speed and efficiency of super-resolution microscopy.
It can prepare 12 samples for imaging in only 4.5 hours, reducing your hands-on time by up to 85% per sample. Our end-to-end product accelerates analysis and interpretation, with modular experimental design, it suits diverse research needs and protocols.
Aplo Scope
Aplo Scope is the flagship innovation behind the Aplo Platform: solid performance in a versatile, easy-to-use microscope. Experience a range of imaging modalities with this highly compact microscope designed to simplify multicolor super-resolution microscopy.
Aplo Scope allows you to start imaging right away, no dark room or optical table is required. Its integrated software enables fixed or live imaging anywhere, and seamless up to 5 color multimode images!
CODI
The entire Aplo platform is powered by ONI’s comprehensive software solution, CODI, which integrates Aplo Flow, Aplo Scope, and powerful cloud-based analysis and reporting tools.
CODI’s intuitive interface makes single-molecule studies accessible to users of all skill levels. Advanced acquisition, analysis, and reporting are all integrated and instantly shareable with other team members.
Live cell imaging
Stage Top Incubator
The Stage Top Incubator is a practical addition for extending live cell studies with the Aplo Scope and is custom designed for this platform. It provides reliable control over temperature, CO2, and humidity, which are crucial for keeping live samples in optimal conditions.
Designed to work seamlessly with the Aplo Scope, it is simple to set up and doesn’t require any hardware changes. It can accommodate both 35 mm dishes and standard microscopy slides making it a flexible tool for various lab needs. This system is available to purchase for both Aplo Scope and Nanoimager platforms and can be easily added to existing installations.
FAQs
How much training is needed to get started using Aplo Scope?
With unparalleled ease-of-use and simplicity, Aplo Scope has been designed to eliminate barriers to entry into the traditionally complex world of super-resolution microscopy and single-molecule imaging, and allow users of any skill level to utilize the instrument within one day of training.
What are the dimensions of Aplo Scope?
The footprint of the microscope body is as small as a standard laptop 220 mm (w) x 421 mm (d) x 242 mm (h), the light engine measures 215 mm (w) x 420 mm (d) x 456 mm (h) and looks like a computer tower. They’re attached to each other by a 1.5 m long cable and, importantly, can be used on any bench or desk without needing a dark room or optical table.
Is Aplo Scope compatible with third-party kits and data analysis tools?
Yes, samples can be stained with any super-resolution microscopy-compatible dye and imaged using Aplo Scope. However, this would require manual acquisition and analysis within CODI, or a third party application. ONI offers end-to-end kits where sample preparation, image acquisition, analysis and reporting are all integrated into a workflow, making imaging a seamless and more automated experience.