Viral Particle Imaging

Challenge

Understand viral particle structure and localization

Viral particles vary greatly in size, ranging from approximately 20 to 300 nm in diameter, which can be below or close to the resolution limit of conventional fluorescence microscopy. Consequently, there are many mechanistic and functional characteristics of viral particles that are yet to be elucidated.

Recently, super-resolution imaging techniques have been employed to study viral particles and their contents at single-molecule level.

Solution with the Nanoimager

Imaging viral particles using dSTORM microscopy

Case Study

Analysing viral particle architecture with dSTORM microscopy

The benefit of super-resolution imaging with dSTORM microscopy is presented in the figure to the right, where the viral surface glycoprotein (red) and cellular host factors incorporated into the particle (green), of a single-virus particle adhered to coverslip, were investigated by labelling with antibodies conjugated to AF647(red) and AF488 (green). The histogram across the viral particle (panel B) shows its width to be around 190 nm, and the cellular host factor accumulating inside the particle. This level of information is not obtainable in a conventional fluorescence widefield image (panel A, inset).

Learn more about the features of the Nanoimager’s NimOS software and the super-resolution microscopy techniques that our microscope supports.

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