Experiment setup
The software has default settings tailored for each variant of the kit. All you need to do is inform the software about your specific reagents, and it will automatically adjust the acquisition settings accordingly. Additionally, users have the flexibility to select and apply specific analysis settings after the imaging process, providing a flexible and efficient experience.

One-click channel mapping
Channel mapping is a simple, one-click step!

One-click system calibration
The system is designed for automatic calibration processes, aimed to enhance data consistency and reproducibility across lanes and chips. These processes include autofocus, automatic-Z-lock, and auto-TIRF, all of which contribute to optimized system performance. The system considers the specific properties of the analyzed sample and automatically determines the appropriate settings. The user simply selects a specific lane and imaging channel where they expect a robust signal for the system calibration, streamlining the overall imaging experience, however, the system also allows for manual settings if the user prefers.

Sample check
The system facilitates a rapid assessment of key features within minutes including the density of EVs in the sample, their distribution and the efficacy of biomarker staining. This step ensures the quality and the reliability of the sample before the full imaging process. If necessary, users have the option to modify the autofocus settings on a per-lane basis, based on the insights they gained from the images. This adaptive approach allows for fine-tuning and optimization in real-time, ultimately contributing to the accuracy and the precision of the imaging results.

Automatic 4-lane acquisition
Users have to flexibility to choose their preferred fields of view (FOV) for imaging or opt for the FOVs recommended by our expert development scientists. The process is streamlined to efficiently and automatically image a 4-lane chip within 90 minutes. Users can access information about the EV count within minutes, thanks to the system’s real-time analysis capabilities. This features provides users with immediate insights, enhancing the overall efficiency and and responsiveness of the imaging workflow.

Summary page & EV mosaic
Upon completion of the 4-lane acquisition, a mosaic summary view offers a visual representation of the biomarker heterogeneity at the single EV level. This feature enables users to swiftly navigate through different EVs of the same positivity class by clicking on next/previous options. Positioned at the bottom of each lane, this mosaic view facilitates a convenient visual comparison of EVs across various conditions. The immediate insights gained from this overview aid in determining the success of the acquisition process.

Custom 1-page EV report
By selecting “Generate Report”, the user can quickly generate a concise 1-page report, featuring three essential, easily comparable metrics. This report provides valuable insight on the quality of your sample, including EV count, biomarker heterogeneity count, and EV size. The metrics are presented at both the population level and according to each biomarker positivity, offering a comprehensive overview of the sample characteristics for efficient analysis and decision-making.