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5 channel hybrid labeling (1 dSTORM plus 4 diffraction limited labels)
Description
1 color dSTORM labeling of mitochondria stained with Tomm20 and AZ647 secondary antibody (yellow) combined with 4 color diffraction limited labeling of lysosomes with LAMP1 and CF583R secondary antibody (red), nucleus stained with Hoescht (blue), cell membrane stained with CellMask Green (gray), and tubulin and CF405L secondary antibody (cyan).
Techniques
5 channel hybrid labeling (1 dSTORM plus 4 diffraction limited labels)
ONI Applications Development team