NimOS Software

NimOS software has been developed to provide users with a range of tools that analyze and present fluorescence microscopy data in a fast and quantitative way

Data acquisition

Acquisition made simple

It is quick and easy to acquire all the data you will ever need using our dedicated Nanoimager software. NimOS is a complete software suite which enables you to control every aspect of your Nanoimager, from laser source selection to exposure time.

Watch your data acquire and then render a super-resolution image in near real-time using our GPU accelerated localization microscopy software. NimOS allows you to tailor your analysis search space to your individual needs.

You can also use a high level programming approach, via our Python command shell, to automate your system to meet your specific scientific application.

With unlimited NimOS licenses you are not tied to doing acquisition and analysis on the same computer. So when you’re sharing a microscope with others your acquisition won’t be held up by your colleagues’ analysis work.

NimOS Support

Our ONI Service Desk contains various support and training materials for NimOS. Simply setup your account and login to access the available materials.

Fluorescence microscopy data analysis

Super-resolution microscopy

  • Quantify molecular clustering
  • Measure colocalization of molecules detected in different colors
  • Distribution of localizations in specific directions
  • Standard deviation of fitted functions, photon counts and other technical details

smFRET microscopy

  • View individual traces or population averages
  • Group traces with distinct behavior
  • 2D histograms of stoichiometry versus fret efficiency for alternating laser excitation (ALEX) mode

Single-particle tracking

  • Includes sptPALM analysis
  • Results generated in seconds
  • Characterization of diffusion including active transport of molecules or particles
  • Estimates size and count of particles tracked in solution
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