Imaging techniques and illumination modes

Choose the best techniques for your research needs

TECHNIQUES

Super-Resolution in many forms

The Nanoimager offers various modes of operation including dSTORM, PALM, smFRET and supports illumination modes from epifluorescence to TIRF. With this range on offer, it's easier than ever to get the most out of fluorescence microscopy, both when imaging fixed samples stained with immunofluorescence protocols or during live-cell imaging.

dSTORM & PALM microscopy

Get up to 10 times better resolution than widefield with dSTORM microscopy and PALM microscopy.

  • 20nm resolution

    Resolve and visualise structures down to 20nm in the XY plane.

  • 3D imaging

    Engage the astigmatic lens and explore the nanoworld in 3D with a Z resolution reaching 50nm.

  • Quantitative Results

    Fluorescence microscopy becomes quantitative with dSTORM and PALM — count individual molecules as well as resolve structures.

Learn more about dSTORM microscopy and PALM microscopy.

dSTORM microscopy

Single-particle tracking

Follow particles at a nanometer scale simultaneously in two channels.

  • Live-cell imaging

    With built in heating control, autofocus and support for microfluidic setups, live-cell imaging couldn’t be easier.

  • Track in four colors, two at once

    With four laser colors, you can track up to four different molecules in one experiment, with two tracked simultaneously.

  • Dedicated tracking analysis

    Our dedicated NimOS software enables automatic particle tracking as well as measurement of diffusion coefficients, particle sizing and concentrations.

Learn more about single-particle tracking.

Single-particle tracking

Single-molecule FRET (smFRET)

Real time nanoscale ruler operates on a 2-10nm range. ONI is the only provider of commercial solutions for smFRET.

  • Insight into molecular interactions

    Determine the spatial proximity of protein molecules so you can identify molecular interactions, binding events and dwell times.

  • Large field of view captures thousands of smFRET events

    Increase experimental throughput by capturing thousands of single molecules at the same time.

  • Dedicated smFRET analysis

    Plot and group individual FRET traces, as well as population averages.

Learn more about single-molecule FRET (smFRET).

Single-molecule FRET (smFRET)

Which super-resolution technique is right for you?

Download our table comparing the options on the Nanoimager

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ILLUMINATION

From Epifluorescence to TIRF microscopy

Enhance the quality of your image by changing the illumination angle at the click of a button.

EPIFLUORESCENCE (EPI)

A parallel beam of light passes directly upwards through the sample. Epifluorescence is preferred for imaging samples over 10µm deep. However, this method does result in higher background signals due to excited molecules outside of the focal plane.

Learn more on epifluorescence microscopy.

Highly inclined and laminated optical sheet (HILO)

The laser is directed at a sharp angle through the sample. HILO affords an imaging depth of up to 10µm, at a signal-to-noise ratio that is almost as good as TIRF microscopy.



Learn more on HILO microscopy.

TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF)

TIRF microscopy is ideal for studying molecules attached to a surface or on a membrane as only a thin, 200nm layer of the sample is excited near the coverslip. Virtually all of the excited molecules are in focus and the background noise is significantly reduced.

Learn more on TIRF microscopy.

EPIFLUORESCENCE (EPI)

A parallel beam of light passes directly upwards through the sample. Epifluorescence is preferred for imaging samples over 10µm deep. However, this method does result in higher background signals due to excited molecules outside of the focal plane.

Learn more on epifluorescence microscopy.

Highly inclined and laminated optical sheet (HILO)

The laser is directed at a sharp angle through the sample. HILO affords an imaging depth of up to 10µm, at a signal-to-noise ratio that is almost as good as TIRF microscopy.



Learn more on HILO microscopy.

TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF)

TIRF microscopy is ideal for studying molecules attached to a surface or on a membrane as only a thin, 200nm layer of the sample is excited near the coverslip. Virtually all of the excited molecules are in focus and the background noise is significantly reduced.

Learn more on TIRF microscopy.

More Information?

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