Imaging Techniques And Illumination Modes

Choose the best techniques for your research needs

TECHNIQUES

Super Resolution in many forms

The Nanoimager offers various modes of operation including dSTORM, PALM, SIM, smFRET and supports illumination modes from TIRF to EPI. With this range on offer, it’s easier than ever to tackle research questions from many angles.

dSTORM & PALM microscopy

Get up to 10 times better resolution than widefield with dSTORM microscopy and PALM microscopy.

  • 20nm resolution

    Resolve and visualise structures down to 20nm in the XY plane.
  • 3D imaging

    Engage the astigmatic lens and explore the nanoworld in 3D imaging up to 50nm Z resolution.
  • Quantitative Results

    Fluorescence microscopy becomes quantitative with dSTORM and PALM - count individual molecules as well as resolve structures.

Learn more about PALM microscopy.

dSTORM microscopy

Single-particle tracking

Follow particles at a nanometer scale simultaneously in two channels.

  • Live cell imaging

    With built in heating control, autofocus and support for microfluidic setups, live cell imaging couldn’t be easier.
  • Track in four colors, two at once

    With four laser colors, you can track up to four different molecules in one experiment, with two tracked simultaneously.
  • Dedicated tracking analysis

    NimOS software enables automatic particle tracking as well as measurement of diffusion coefficients, particle sizing and concentrations.
Single-particle tracking

SIM & confocal microscopy

Easier to use than dSTORM, SIM microscopy offers accessible super-resolution and offers twice the resolution of widefield.

  • Fast and easy super-resolution

    Supported by Z-stack imaging and timelapses, SIM imaging is the elegant, fast and robust super-resolution technique.
  • For live and fixed samples

    From fluorescent proteins to immunolabelling, sample preparation is no longer a limit.
  • Fluorophore flexibility

    Wide selection of fluorophores make the technique robust and versatile.

Learn more about SIM microscopy and confocal microscopy.

SIM and confocal microscopy

Single-molecule FRET (smFRET)

Real time nanoscale ruler operates on a 1-10nm range. ONI is the only provider of commercial solutions for smFRET.

  • Insight into molecular interactions

    Determine the spatial proximity of protein molecules so you can identify molecular interactions, binding events and dwell times.
  • Large field of view captures thousands of smFRET events

    Increase experimental throughput by capturing thousands of single molecules at the same time.
  • Dedicated smFRET analysis

    Plot and group individual FRET traces, as well as population averages.

Learn more about single-molecule FRET (smFRET).

Single-molecule FRET (smFRET)

ILLUMINATION

From Epifluorescence to TIRF microscopy

Enhance the quality of your image by changing the illumination angle at the click of a button.

EPIFLUORESCENCE (EPI)

Epifluorescence

A parallel beam of light passes directly upwards through the sample. Epifluorescence is preferred for imaging samples over 10µm deep. However, this method does result in higher background signals due to excited molecules outside of the focal plane.

Learn more about epifluorescence microscopy.

TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF)

TIRF microscopy

TIRF microscopy is ideal for studying molecules attached to a surface or on a membrane as only a thin, 200nm layer of the sample is excited near the coverslip. Virtually all of the excited molecules are in focus and the background noise is significantly reduced.

Learn more about TIRF microscopy.

Highly inclined and laminated optical sheet (HILO)

Highly inclined and laminated optical sheet

The laser is directed at a sharp angle through the sample. HILO affords an imaging depth of up to 10µm, at a signal-to-noise ratio that is almost as good as TIRF microscopy.

Learn more about HILO microscopy.

EPIFLUORESCENCE (EPI)

Epifluorescence

A parallel beam of light passes directly upwards through the sample. Epifluorescence is preferred for imaging samples over 10µm deep. However, this method does result in higher background signals due to excited molecules outside of the focal plane.

TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF)

TIRF microscopy

TIRF microscopy is ideal for studying molecules attached to a surface or on a membrane as only a thin, 200nm layer of the sample is excited near the coverslip. Virtually all of the excited molecules are in focus and the background noise is significantly reduced.

Highly inclined and laminated optical sheet (HILO)

Highly inclined and laminated optical sheet

The laser is directed at a sharp angle through the sample. HILO affords an imaging depth of up to 10µm, at a signal-to-noise ratio that is almost as good as TIRF microscopy.

Need advice on techniques?

Our team of scientists are waiting to help with your questions