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NimOS: an elegant solution

A common problem across single-molecule biology is the difficulty of interpreting complex results. To support researchers, the Nanoimager software suite 'NimOS' contains a range of features that analyze and present data in an accessible way, as well as providing quantitative conclusions about the data.

Acquisition made simple

It is quick and easy to acquire all the data you will ever need using our dedicated Nanoimager software. NimOS is a complete software suite which enables you to control every aspect of your Nanoimager, from laser source selection to exposure time.

Watch your data acquire and then render a super resolution image in near real time using our GPU accelerated localization software. NimOS allows you to tailor your analysis search space to your individual needs.

You can also use a high level programming approach, via our Python command shell, to automate your system to meet your specific scientific application.

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Quantitative single-molecule analysis


  • Quantify molecular clustering
  • Measure colocalization of molecules detected in different colors
  • Distribution of localizations in specific directions
  • Standard deviation of fitted functions, photon counts and other technical details


  • View individual traces or population averages
  • Group traces with distinct behavior
  • 2D histograms of stoichiometry versus FRET efficiency for alternating laser excitation (ALEX) mode

Single-particle tracking

  • Including sptPALM
  • Generated in seconds
  • Characterization of diffusion and active transport of molecules or vesicles
  • Estimated size and counting of particles tracked in solution

Real-time rendering

We generate super-resolved images by localizing single molecules in real time using sCMOS-optimized localization on the GPU. It is also possible to change viewing options – localization filtering, enlargement of features and colour – in real time. We strive to make all our viewing and analysis tools intuitive for first-time users.

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