Assess EV morphology
Single molecule localization microscopy used in combination with the appropriate fluorescently labeled probes, helps assess the integrity and morphology of EV populations and single EVs after purification. ONI’s pan-EV stain in combination with cluster-based analysis on our CODI cloud platform, allows reporting of morphological features of the EV, including size and circularity.
The EV Profiler Kit V2 is powered by ONI’s CODI cloud software, which enables reporting of EV morphological features based on clustering of single molecule data. The different EV morphologies within heterogeneous EV populations can be determined with features such as diameter and circularity; two examples are shown with images superimposed to the graph with A representing a smaller and less circular EV, and B representing a larger more circular EV.