ONI is supporting the global effort into research and diagnostics to tackle the COVID-19 pandemic, we are currently directing all collaborative and R&D efforts towards this goal.
If you have ideas about how we can support your COVID-19 research efforts, please get in touch.
These super-resolution images show the effect of Ebola virus-like particles on the actin cytoskeleton. The virus particles co-localize with the disrupted actin network. This reveals how exactly the virus is able to replicate in its host, providing researchers with insights into possible targets for therapeutics. The data was acquired in collaboration with Professor Stephan Becker, a world class virologist at Philipps-Universität, Marburg.
Single-particle tracking of influenza HA (HA-Dendra) inside HEK293T cells, Prof. Salvatore Chiantia, University of Potsdam.
Viruses hijack and exploit their hosts’ cells to replicate and spread. The packaging of new viral particles occurs at the plasma membrane of infected cells. We can understand these assembly sites by visualizing the motion and interactions of key proteins, such as those forming the viral envelope. In this case we followed single HA proteins, part of the influenza viral envelope, with Prof. Salvo Chianti of University of Potsdam.
Imaging cells after infection with a virus can reveal much about the viral mechanism of action. In 2014, Mousnier et al showed how human rhinovirus affects Golgi disruption and protein secretion (doi: 10.1128/JVI.01170-14). We visited Queen’s University Belfast to replicate some of these experiments. Note how the Golgi is left intact in the uninfected cells.
Rhinovirus (viral protein 2C-AF647) infects HeLa cells and disrupts the Golgi apparatus (GM130-AF488). Acquired with Drs A Mousnier and G Schroeder, Queen’s University Belfast.
The Nanoimager has been validated in biosafety cabinets as well as on the lab bench, has a tiny footprint and is remotely operable. The ideal platform for containment labs.